PMID:9463391
| Citation |
Janosi, L, Mottagui-Tabar, S, Isaksson, LA, Sekine, Y, Ohtsubo, E, Zhang, S, Goon, S, Nelken, S, Shuda, M and Kaji, A (1998) Evidence for in vivo ribosome recycling, the fourth step in protein biosynthesis. EMBO J. 17:1141-51 |
|---|---|
| Abstract |
Ribosome recycling factor (RRF) catalyzes the fourth step of protein synthesis in vitro: disassembly of the post-termination complex of ribosomes, mRNA and tRNA. We now report the first in vivo evidence of RRF function using 12 temperature-sensitive Escherichia coli mutants which we isolated in this study. At non-permissive temperatures, most of the ribosomes remain on mRNA, scan downstream from the termination codon, and re-initiate translation at various sites in all frames without the presence of an initiation codon. Re-initiation does not occur upstream from the termination codon nor beyond a downstream initiation signal. RRF inactivation was bacteriostatic in the growing phase and bactericidal during the transition between the stationary and growing phase, confirming the essential nature of the fourth step of protein synthesis in vivo. |
| Links |
PubMed PMC1170462 Online version:10.1093/emboj/17.4.1141 |
| Keywords |
Alleles; Amino Acid Sequence; Amino Acid Substitution/genetics; Bacterial Proteins/biosynthesis; Bacterial Proteins/genetics; Bacterial Proteins/isolation & purification; Base Sequence; Chromosomes, Bacterial/genetics; Codon/physiology; Escherichia coli/metabolism; Escherichia coli/physiology; Molecular Sequence Data; Mutagenesis; Peptide Chain Initiation, Translational/genetics; Peptide Chain Termination, Translational/genetics; Phenotype; Proteins; Ribosomal Proteins; Ribosomes/metabolism; Temperature |
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