PMID:9440535
| Citation |
Zenno, S, Kobori, T, Tanokura, M and Saigo, K (1998) Conversion of NfsA, the major Escherichia coli nitroreductase, to a flavin reductase with an activity similar to that of Frp, a flavin reductase in Vibrio harveyi, by a single amino acid substitution. J. Bacteriol. 180:422-5 |
|---|---|
| Abstract |
NfsA is the major oxygen-insensitive nitroreductase of Escherichia coli, similar in amino acid sequence to Frp, a flavin reductase of Vibrio harveyi. Here, we show that a single amino acid substitution at position 99, which may destroy three hydrogen bonds in the putative active center, transforms NfsA from a nitroreductase into a flavin reductase that is as active as the authentic Frp and a tartrazine reductase that is 30-fold more active than wild-type NfsA. |
| Links | |
| Keywords |
Amino Acid Sequence; Escherichia coli/enzymology; FMN Reductase; Follistatin-Related Proteins; Glycoproteins/chemistry; Glycoproteins/metabolism; Hydrogen Bonding; Kinetics; Molecular Sequence Data; NADH, NADPH Oxidoreductases/biosynthesis; NADH, NADPH Oxidoreductases/chemistry; NADH, NADPH Oxidoreductases/metabolism; Nitroreductases/metabolism; Sequence Homology, Amino Acid; Vibrio/enzymology |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.
