PMID:9393863
| Citation |
Correll, CC, Freeborn, B, Moore, PB and Steitz, TA (1997) Metals, motifs, and recognition in the crystal structure of a 5S rRNA domain. Cell 91:705-12 |
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| Abstract |
Two new RNA structures portray how non-Watson-Crick base pairs and metal ions can produce a unique RNA shape suitable for recognition by proteins. The crystal structures of a 62 nt domain of E. coli 5S ribosomal RNA and a duplex dodecamer encompassing an internal loop E have been determined at 3.0 and 1.5 A, respectively. This loop E region is distorted by three "cross-strand purine stacks" and three novel, water-mediated noncanonical base pairs and stabilized by a four metal ion zipper. These features give its minor groove a unique hydrogen-bonding surface and make the adjacent major groove wide enough to permit recognition by the ribosomal protein L25, which is expected to bind to this surface. |
| Links | |
| Keywords |
Cross-Linking Reagents; Crystallography; Escherichia coli/chemistry; Ions; Metals/chemistry; Nucleic Acid Conformation; Purines/chemistry; RNA, Ribosomal, 5S/chemistry |
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