PMID:9391106
| Citation |
Kim, SR, Maenhaut-Michel, G, Yamada, M, Yamamoto, Y, Matsui, K, Sofuni, T, Nohmi, T and Ohmori, H (1997) Multiple pathways for SOS-induced mutagenesis in Escherichia coli: an overexpression of dinB/dinP results in strongly enhancing mutagenesis in the absence of any exogenous treatment to damage DNA. Proc. Natl. Acad. Sci. U.S.A. 94:13792-7 |
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| Abstract |
dinP is an Escherichia coli gene recently identified at 5.5 min of the genetic map, whose product shows a similarity in amino acid sequence to the E. coli UmuC protein involved in DNA damage-induced mutagenesis. In this paper we show that the gene is identical to dinB, an SOS gene previously localized near the lac locus at 8 min, the function of which was shown to be required for mutagenesis of nonirradiated lambda phage infecting UV-preirradiated bacterial cells (termed lambdaUTM for lambda untargeted mutagenesis). A newly constructed dinP null mutant exhibited the same defect for lambdaUTM as observed previously with a dinB::Mu mutant, and the defect was complemented by plasmids carrying dinP as the only intact bacterial gene. Furthermore, merely increasing the dinP gene expression, without UV irradiation or any other DNA-damaging treatment, resulted in a strong enhancement of mutagenesis in F'lac plasmids; at most, 800-fold increase in the G6-to-G5 change. The enhanced mutagenesis did not depend on recA, uvrA, or umuDC. Thus, our results establish that E. coli has at least two distinct pathways for SOS-induced mutagenesis: one dependent on umuDC and the other on dinB/P. |
| Links | |
| Keywords |
Bacterial Proteins/genetics; Base Sequence; DNA Damage; DNA, Bacterial/genetics; DNA-Directed DNA Polymerase; Escherichia coli/genetics; Escherichia coli Proteins; Gene Expression; Genes, Bacterial; Genetic Complementation Test; Mutagenesis; Plasmids/genetics; Promoter Regions, Genetic; SOS Response (Genetics)/genetics |
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