PMID:9387241
| Citation |
Andrews, SC, Berks, BC, McClay, J, Ambler, A, Quail, MA, Golby, P and Guest, JR (1997) A 12-cistron Escherichia coli operon (hyf) encoding a putative proton-translocating formate hydrogenlyase system. Microbiology (Reading, Engl.) 143 ( Pt 11):3633-47 |
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| Abstract |
The nucleotide sequence has been determined for a twelve-gene operon of Escherichia coli designated the hyf operon (hyfABCDEFGHIR-focB). The hyf operon is located at 55.8-56.0 min and encodes a putative nine-subunit hydrogenase complex (hydrogenase four or Hyf), a potential formate- and sigma 54-dependent transcriptional activator, HyfR (related to FhlA), and a possible formate transporter, FocB (related to FocA). Five of the nine Hyf-complex subunits are related to subunits of both the E. coli hydrogenase-3 complex (Hyc) and the proton-translocating NADH:quinone oxidoreductases (complex I and Nuo), whereas two Hyf subunits are related solely to NADH:quinone oxidoreductase subunits. The Hyf components include a predicted 523 residue [Ni-Fe] hydrogenase (large subunit) with an N-terminus (residues 1-170) homologous to the 30 kDa or NuoC subunit of complex I. It is proposed that Hyf, in conjunction with formate dehydrogenase H (Fdh-H), forms a hitherto unrecognized respiration-linked proton-translocating formate hydrogenlyase (FHL-2). It is likely that HyfR acts as a formate-dependent regulator of the hyf operon and that FocB provides the Hyf complex with external formate as substrate. |
| Links | |
| Keywords |
Amino Acid Sequence; Bacterial Proteins/chemistry; Bacterial Proteins/genetics; Escherichia coli/enzymology; Escherichia coli/genetics; Formate Dehydrogenases/chemistry; Formate Dehydrogenases/genetics; Genes, Bacterial/genetics; Hydrogenase/chemistry; Hydrogenase/genetics; Membrane Proteins/chemistry; Membrane Proteins/genetics; Models, Chemical; Molecular Sequence Data; Multienzyme Complexes/chemistry; Multienzyme Complexes/genetics; Open Reading Frames/genetics; Operon/genetics; Protein Structure, Secondary; Protons; Restriction Mapping; Sequence Alignment; Sequence Analysis, DNA; Sequence Homology, Amino Acid |
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