PMID:9383472
| Citation |
Yap, LP, Stehlin, C and Musier-Forsyth, K (1995) Use of semi-synthetic transfer RNAs to probe molecular recognition by Escherichia coli proline-tRNA synthetase. Chem. Biol. 2:661-6 |
|---|---|
| Abstract |
The attachment of specific amino acids to the 3'-end of cognate transfer of RNAs (tRNAs) is catalyzed by a class of enzymes known as aminoacyl-tRNA synthetases (aaRS). We have previously demonstrated that Escherichia coli proline-tRNA synthetase (ProRS) can aminoacylate semi-synthetic tRNAs prepared by annealing two RNA oligonucleotides. We set out to examine the factors that are important in selective recognition of tRNAPro by ProRS, using semi-synthetic tRNAs and full-length tRNA transcripts. |
| Links | |
| Keywords |
Amino Acids/chemistry; Amino Acyl-tRNA Synthetases/metabolism; Escherichia coli/enzymology; Mutagenesis, Site-Directed; Nucleic Acid Conformation; Plasmids/genetics; RNA Probes/chemistry; RNA, Transfer, Pro/chemistry; Sequence Deletion; Transcription, Genetic |
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