PMID:9383196
| Citation |
Botello, E and Jiménez-Sánchez, A (1997) A temperature upshift induces initiation of replication at oriC on the Escherichia coli chromosome. Mol. Microbiol. 26:133-44 |
|---|---|
| Abstract |
A temperature upshift of 10 or more degrees in the growth temperature of a bacterial culture causes induction of extra rounds of chromosome replication. This heat-induced replication (HIR) initiates at oriC, is transitory, requires RNase H1 and RecA proteins and requires neither RNA polymerase activity nor de novo protein synthesis. The number of origins activated by heat is growth rate and temperature differential dependent. An origin activation higher than 20% increases the DNA:mass ratio around twofold, and this value is kept constant for the subsequent generations of growth at 41 C. We have also shown that HIR is neither related to SDR nor induced by the heat shock response. We suggest that a thermodynamic alteration of oriC structure or of membrane fluidity could explain the observed HIR. |
| Links | |
| Keywords |
Bacterial Proteins/biosynthesis; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; DNA Replication; DNA, Bacterial/biosynthesis; Escherichia coli/genetics; Escherichia coli/growth & development; Escherichia coli/metabolism; Escherichia coli Proteins; Heat-Shock Proteins/genetics; Heat-Shock Proteins/metabolism; Heat-Shock Response; Hot Temperature; Isopropyl Thiogalactoside/pharmacology; Lac Repressors; Plasmids; Rec A Recombinases/metabolism; Replication Origin; Repressor Proteins/genetics; Ribonuclease H/metabolism; Rifampin/pharmacology; Sigma Factor/genetics; Sigma Factor/metabolism; Transcription Factors |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.