PMID:9379062
| Citation |
MacKenzie-Graham, AJ, Pribyl, TM, Kim, S, Porter, VR, Campagnoni, AT and Voskuhl, RR (1997) Myelin protein expression is increased in lymph nodes of mice with relapsing experimental autoimmune encephalomyelitis. J. Immunol. 159:4602-10 |
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| Abstract |
Myelin proteins had been thought to be sequestered behind the blood-brain barrier. Recently, however, myelin proteins have been found to be expressed in lymphoid tissues. The myelin basic protein (MBP) gene is embedded within a larger transcription unit called the golli-MBP gene. This larger gene encodes both the "classic" MBPs as well as the structurally related golli-MBPs. In this study, golli-MBP expression in lymph nodes was examined in four different models of relapsing experimental autoimmune encephalomyelitis (rEAE). Disease in these rEAE models was induced by the adoptive transfer of T lymphocytes specific for 18.5-kDa MBP, MBP peptide 83-102, or PLP peptide 139-151 in the SJL/J mouse and the adoptive transfer of T lymphocytes specific for MBP peptide Ac1-9 in the (SJL/J x PL/J)F1 mouse. In all four models, expression of golli-MBP BG21 mRNA was increased two- to fivefold in lymph nodes of mice 45 to 60 days post-transfer. Immunohistochemical analysis indicated that expression occurred principally in macrophages within lymph nodes. Endogenous golli-MBP epitopes within lymph node cells stimulated "classic" MBP 1-44-specific T lymphocytes, and this stimulatory ability resided within the adherent lymph node cell population. An increase in myelin protein expression within lymph nodes during rEAE has implications with regard to intra- and intermolecular epitope spreading. This is the first report describing an increase in target autoantigen expression within lymphoid tissue during an autoimmune disease. |
| Links | |
| Keywords |
Amino Acid Sequence; Animals; Autoantigens/biosynthesis; Autoantigens/genetics; Autoantigens/immunology; Encephalomyelitis, Autoimmune, Experimental/immunology; Encephalomyelitis, Autoimmune, Experimental/metabolism; Encephalomyelitis, Autoimmune, Experimental/pathology; Female; Gene Expression Regulation; Lymph Nodes/immunology; Lymph Nodes/metabolism; Mice; Molecular Sequence Data; Myelin Basic Protein/biosynthesis; Myelin Basic Protein/genetics; Myelin Basic Protein/immunology; Recurrence |
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