PMID:9352941
| Citation |
Ebel, W, Vaughn, GJ, Peters, HK 3rd and Trempy, JE (1997) Inactivation of mdoH leads to increased expression of colanic acid capsular polysaccharide in Escherichia coli. J. Bacteriol. 179:6858-61 |
|---|---|
| Abstract |
Capsule gene (cps) expression, which normally occurs at low levels in Escherichia coli lon+ cells, increased 38-fold in lon+ cells carrying a Tn10::delta kan insertion mapping to 24 min on the E. coli chromosome. Null mutations in rcsA, rcsB, or rcsC abolished the effect of the Tn10::delta kan insertion. Sequencing of both sides of the Tn10::delta kan insertion localized the insertion to the previously reported mdoH gene, which encodes a protein involved in biosynthesis of membrane-derived oligosaccharides (MDOs). A model suggesting that the periplasmic levels of MDOs act to signal RcsC to activate cps expression is proposed. |
| Links | |
| Keywords |
ATP-Dependent Proteases; Bacterial Capsules/biosynthesis; Bacterial Proteins/genetics; Escherichia coli/genetics; Escherichia coli Proteins; Gene Expression Regulation, Bacterial; Heat-Shock Proteins/genetics; Membrane Proteins/genetics; Multienzyme Complexes; Mutagenesis, Insertional; Osmotic Pressure; Phosphoprotein Phosphatases; Polysaccharides/biosynthesis; Protease La; Protein Kinases; Serine Endopeptidases/genetics; Transcription Factors/genetics |
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