PMID:9344868
| Citation |
Chilukuri, LN, Fortes, PA and Bartlett, DH (1997) High pressure modulation of Escherichia coli DNA gyrase activity. Biochem. Biophys. Res. Commun. 239:552-6 |
|---|---|
| Abstract |
Elevated pressures greater than 551 bar were found to inhibit the DNA supercoiling activity of Escherichia coli DNA gyrase. A large fraction of the inhibitory effect could be reproduced by preincubation of the enzyme at high pressure prior to enzymatic assay at 1 bar. It is proposed that elevated pressure influences gyrase structure, most likely by promoting the dissociation of its subunits, however, it is also possible that effects on enzyme activity exist. |
| Links |
PubMed Online version:10.1006/bbrc.1997.7503 |
| Keywords |
Atmospheric Pressure; DNA Topoisomerases, Type II/metabolism; DNA, Superhelical/metabolism; Electrophoresis, Agar Gel; Enzyme Activation; Escherichia coli/enzymology; Plasmids/metabolism; Substrate Specificity |
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