PMID:9341229
| Citation |
Kerr, KM and Hedstrom, L (1997) The roles of conserved carboxylate residues in IMP dehydrogenase and identification of a transition state analog. Biochemistry 36:13365-73 |
|---|---|
| Abstract |
IMP dehydrogenase (IMPDH) catalyzes the oxidation of IMP to XMP with the concomitant reduction of NAD+; the enzyme is activated by K+. This reaction is the rate-limiting step in de novo guanine nucleotide biosynthesis. In order to identify functionally important residues in IMPDH, including those involved in substrate and K+ binding, we have mutated 11 conserved Asp and Glu residues to Ala in Escherichia coli IMPDH. The values of kcat, Km, and Ki for GMP, XMP, mizoribine 5'-monophosphate (MMP), and beta-methylene-tiazofurin adenine dinucleotide (TAD) were determined. Five of these mutations caused a significant change (>/=10-fold) in one of these parameters. The Asp248 --> Ala mutation caused 100-fold decrease in the value of kcat and a 25-fold increase in the value of Kii for TAD; these observations suggest that Asp248 is in the NAD+ binding site. The Asp338 --> Ala mutation caused a 600-fold decrease in the value of kcat, but only a 5-10-fold increase in the values of Km for IMP and Kis for IMP analogs, suggesting that Asp338 may be involved in acid-base catalysis as well as IMP binding. The remaining three residues, Asp13, Asp50, and Glu469, appear to be involved in K+ activation; these residues may be ligands at one or more K+ binding sites. Interestingly, changes in the values of Ki for MMP correlate with changes in kcat/KmKm of IMPDH, while no such correlation is observed for GMP, XMP, and TAD. This observation indicates that MMP is a transition state analog for the IMPDH reaction. |
| Links |
PubMed Online version:10.1021/bi9714161 |
| Keywords |
Amino Acid Sequence/genetics; Animals; Aspartic Acid/genetics; Binding Sites/genetics; Carboxylic Acids/chemistry; Conserved Sequence/genetics; Cross-Linking Reagents; Enzyme Activation/drug effects; Enzyme Activation/genetics; Escherichia coli/enzymology; Escherichia coli/genetics; Ethyldimethylaminopropyl Carbodiimide; Glutamic Acid/genetics; Humans; IMP Dehydrogenase/antagonists & inhibitors; IMP Dehydrogenase/chemistry; IMP Dehydrogenase/genetics; Mutagenesis, Site-Directed; Protein Conformation; Ribonucleosides/pharmacology |
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