PMID:9326588
| Citation |
Shiba, T, Tsutsumi, K, Yano, H, Ihara, Y, Kameda, A, Tanaka, K, Takahashi, H, Munekata, M, Rao, NN and Kornberg, A (1997) Inorganic polyphosphate and the induction of rpoS expression. Proc. Natl. Acad. Sci. U.S.A. 94:11210-5 |
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| Abstract |
Inorganic polyphosphate [poly(P)] levels in Escherichia coli were reduced to barely detectable concentrations by expression of the plasmid-borne gene for a potent yeast exopolyphosphatase [poly(P)ase]. As a consequence, resistance to H2O2 was greatly diminished, particularly in katG (catalase HPI) mutants, implying a major role for the other catalase, the stationary-phase KatE (HPII), which is rpoS dependent. Resistance was restored to wild-type levels by complementation with plasmids expressing ppk, the gene for PPK [the polyphosphate kinase that generates poly(P)]. Induction of expression of both katE and rpoS (the stationary-phase sigma factor) was prevented in cells in which the poly(P)ase was overproduced. Inasmuch as this inhibition by poly(P)ase did not affect the levels of the stringent-response guanosine nucleotides (pppGpp and ppGpp) and in view of the capacity of additional rpoS expression to suppress the poly(P)ase inhibition of katE expression, a role is proposed for poly(P) in inducing the expression of rpoS. |
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| Keywords |
Acid Anhydride Hydrolases/biosynthesis; Acid Anhydride Hydrolases/genetics; Bacterial Proteins/biosynthesis; Catalase/biosynthesis; Escherichia coli/drug effects; Escherichia coli/genetics; Escherichia coli/metabolism; Gene Expression Regulation, Bacterial; Gene Transfer Techniques; Genetic Complementation Test; Hydrogen Peroxide/pharmacology; Kinetics; Phosphotransferases (Phosphate Group Acceptor)/biosynthesis; Phosphotransferases (Phosphate Group Acceptor)/genetics; Plasmids; Polyphosphates/metabolism; Recombinant Fusion Proteins/biosynthesis; Saccharomyces cerevisiae/enzymology; Saccharomyces cerevisiae/genetics; Sigma Factor/biosynthesis; Suppression, Genetic; Transcription, Genetic |
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