PMID:9308969
| Citation |
Harris, RS, Feng, G, Ross, KJ, Sidhu, R, Thulin, C, Longerich, S, Szigety, SK, Winkler, ME and Rosenberg, SM (1997) Mismatch repair protein MutL becomes limiting during stationary-phase mutation. Genes Dev. 11:2426-37 |
|---|---|
| Abstract |
Postsynthesis mismatch repair is an important contributor to mutation avoidance and genomic stability in bacteria, yeast, and humans. Regulation of its activity would allow organisms to regulate their ability to evolve. That mismatch repair might be down-regulated in stationary-phase Escherichia coli was suggested by the sequence spectrum of some stationary-phase ("adaptive") mutations and by the observations that MutS and MutH levels decline during stationary phase. We report that overproduction of MutL inhibits mutation in stationary phase but not during growth. MutS overproduction has no such effect, and MutL overproduction does not prevent stationary-phase decline of either MutS or MutH. These results imply that MutS and MutH decline to levels appropriate for the decreased DNA synthesis in stationary phase, whereas functional MutL is limiting for mismatch repair specifically during stationary phase. Modulation of mutation rate and genetic stability in response to environmental or developmental cues, such as stationary phase and stress, could be important in evolution, development, microbial pathogenicity, and the origins of cancer. |
| Links | |
| Keywords |
Adenosine Triphosphatases; Bacterial Proteins/genetics; DNA Repair/genetics; DNA, Bacterial/genetics; Escherichia coli/genetics; Escherichia coli Proteins; Mutation |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.