PMID:9275163

Citation	Carmona, M, Claverie-Martin, F and Magasanik, B (1997) DNA bending and the initiation of transcription at sigma54-dependent bacterial promoters. Proc. Natl. Acad. Sci. U.S.A. 94:9568-72
Abstract	We have examined the effects on transcription initiation of promoter and enhancer strength and of the curvature of the DNA separating these entities on wild-type and mutated enhancer-promoter regions at the Escherichia coli sigma54-dependent promoters glnAp2 and glnHp2 on supercoiled and linear DNA. Our results, together with previously reported observations by other investigators, show that the initiation of transcription on linear DNA requires a single intrinsic or induced bend in the DNA, as well as a promoter with high affinity for sigma54-RNA polymerase, but on supercoiled DNA requires either such a bend or a high affinity promoter but not both. The examination of the DNA sequence of all nif gene activator- or nitrogen regulator I-sigma54 promoters reveals that those lacking a binding site for the integration host factor have an intrinsic single bend in the DNA separating enhancer from promoter.
Links	PubMed PMC23220
Keywords	Bacterial Proteins/genetics; Computer Simulation; DNA, Bacterial/genetics; DNA-Binding Proteins; DNA-Directed RNA Polymerases/genetics; Escherichia coli/genetics; Escherichia coli Proteins; Models, Molecular; RNA Polymerase Sigma 54; Sigma Factor/genetics; Transcription, Genetic
edit table

Significance

You can help EcoliWiki by summarizing why this paper is useful

You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.

Add links to pages that link here (e.g. gene, product, method pages)

See Help:References for how to manage references in EcoliWiki.