PMID:9271123
| Citation |
Connolly, L, De Las Penas, A, Alba, BM and Gross, CA (1997) The response to extracytoplasmic stress in Escherichia coli is controlled by partially overlapping pathways. Genes Dev. 11:2012-21 |
|---|---|
| Abstract |
The activity of the alternate sigma-factor sigmaE of Escherichia coli is induced by several stressors that lead to the extracytoplasmic accumulation of misfolded or unfolded protein. The sigmaE regulon contains several genes, including that encoding the periplasmic protease DegP, whose products are thought to be required for maintaining the integrity of the cell envelope because cells lacking sigmaE are sensitive to elevated temperature and hydrophobic agents. Selection of multicopy suppressors of the temperature-sensitive phenotype of cells lacking sigmaE revealed that overexpression of the lipoprotein NlpE restored high temperature growth to these cells. Overexpression of NlpE has been shown previously to induce DegP synthesis by activating the Cpx two-component signal transduction pathway, and suppression of the temperature-sensitive phenotype by NlpE was found to be dependent on the Cpx proteins. In addition, a constitutively active form of the CpxA sensor/kinase also fully suppressed the temperature-sensitive defect of cells lacking sigmaE. DegP was found to be necessary, but not sufficient, for suppression. Activation of the Cpx pathway has also been shown to alleviate the toxicity of several LamB mutant proteins. Together, these results reveal the existence of two partially overlapping regulatory systems involved in the response to extracytoplasmic stress in E. coli. |
| Links | |
| Keywords |
Bacterial Outer Membrane Proteins/genetics; Bacterial Proteins/physiology; Escherichia coli/genetics; Escherichia coli/growth & development; Escherichia coli Proteins; Gene Expression Regulation, Bacterial/physiology; Genes, Bacterial/physiology; Genes, Suppressor/genetics; Heat-Shock Proteins; Heat-Shock Response/genetics; Lipoproteins/genetics; Open Reading Frames/genetics; Periplasmic Proteins; Phenotype; Protein Kinases; Regulon/physiology; Sequence Analysis, DNA; Serine Endopeptidases/genetics; Serine Endopeptidases/physiology; Sigma Factor/genetics; Sigma Factor/physiology; Signal Transduction/genetics; Transcription Factors/genetics; Transcription Factors/physiology |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.