PMID:9245691
| Citation |
Takada, A, Wachi, M, Kaidow, A, Takamura, M and Nagai, K (1997) DNA binding properties of the hfq gene product of Escherichia coli. Biochem. Biophys. Res. Commun. 236:576-9 |
|---|---|
| Abstract |
We found that plasmids isolated by alkaline-lysis method occasionally showed abnormal mobility on agarose gel electrophoresis. This abnormality was not detected after phenol extraction of plasmids, indicating that it was caused by proteinous factor(s). A 15-kDa protein was found in the plasmid preparations on SDS-PAGE. The sequence of eighteen N-terminal amino acid residues of the 15-kDa protein was identical with the host factor I (HF-I) encoded by the hfq gene at 95 min on the Escherichia coli chromosome map, which is known to be required for bacteriophage Qbeta replication. The HF-I protein was purified and in vitro DNA binding experiment was carried out. HF-I bound to both supercoiled DNA and linear DNA and the binding of HF-I seemed to be sequence-nonspecific. |
| Links |
PubMed Online version:10.1006/bbrc.1997.7013 |
| Keywords |
Amino Acid Sequence; Carrier Proteins/chemistry; Carrier Proteins/metabolism; Chromatography; DNA/metabolism; DNA-Binding Proteins/metabolism; Electrophoresis, Agar Gel; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Escherichia coli Proteins; Host Factor 1 Protein; Integration Host Factors; Molecular Sequence Data; Plasmids |
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