PMID:9244308
| Citation |
Riechmann, L and Holliger, P (1997) The C-terminal domain of TolA is the coreceptor for filamentous phage infection of E. coli. Cell 90:351-60 |
|---|---|
| Abstract |
Filamentous bacteriophages infecting gram-negative bacteria display tropism for a variety of pilus structures. However, the obligatory coreceptor of phage infection, postulated from genetic studies, has remained elusive. Here we identify the C-terminal domain of the periplasmic protein TolA as the coreceptor for infection of Escherichia coli by phage fd and the N-terminal domain of the phage minor coat protein g3p as its cognate ligand. The neighboring g3p domain binds the primary receptor of phage infection, the F pilus, and blocks TolA binding in its absence. Contact with the pilus releases this blockage during infection. Our findings support a sequential two-way docking mechanism for phage infection, analogous to infection pathways proposed for a range of eukaryotic viruses including herpes simplex, adenoviruses, and also lentiviruses like HIV-1. |
| Links | |
| Keywords |
Amino Acid Sequence; Bacterial Proteins/chemistry; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Bacteriophage M13/chemistry; Bacteriophage M13/metabolism; Capsid Proteins; DNA-Binding Proteins/chemistry; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Enzyme-Linked Immunosorbent Assay; Escherichia coli/chemistry; Escherichia coli/virology; Escherichia coli Proteins; Fimbriae, Bacterial/metabolism; Fimbriae, Bacterial/virology; Gene Deletion; Inovirus/chemistry; Inovirus/metabolism; Magnetic Resonance Spectroscopy; Membrane Proteins/chemistry; Membrane Proteins/genetics; Membrane Proteins/metabolism; Molecular Sequence Data; Mutagenesis/physiology; Protein Binding/physiology; Protein Structure, Tertiary; Recombinant Proteins/metabolism; Viral Fusion Proteins/chemistry; Viral Fusion Proteins/genetics; Viral Fusion Proteins/metabolism |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.