PMID:9211896
| Citation |
Liu, MY, Gui, G, Wei, B, Preston, JF 3rd, Oakford, L, Yüksel, U, Giedroc, DP and Romeo, T (1997) The RNA molecule CsrB binds to the global regulatory protein CsrA and antagonizes its activity in Escherichia coli. J. Biol. Chem. 272:17502-10 |
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| Abstract |
The RNA-binding protein CsrA (carbon storage regulator) is a new kind of global regulator, which facilitates specific mRNA decay. A recombinant CsrA protein containing a metal-binding affinity tag (CsrA-H6) was purified to homogeneity and authenticated by N-terminal sequencing, matrix-assisted laser desorption/ionization time of flight mass spectrometry, and other studies. This protein was entirely contained within a globular complex of approximately 18 CsrA-H6 subunits and a single approximately 350-nucleotide RNA, CsrB. cDNA cloning and nucleotide sequencing revealed that the csrB gene is located downstream from syd in the 64-min region of the Escherichia coli K-12 genome and contains no open reading frames. The purified CsrA-CsrB ribonucleoprotein complex was active in regulating glg (glycogen biosynthesis) gene expression in vitro, as was the RNA-free form of the CsrA protein. Overexpression of csrB enhanced glycogen accumulation in E. coli, a stationary phase process that is repressed by CsrA. Thus, CsrB RNA is a second component of the Csr system, which binds to CsrA and antagonizes its effects on gene expression. A model for regulatory interactions in Csr is presented, which also explains previous observations on the homologous system in Erwinia carotovora. A highly repeated nucleotide sequence located within predicted stem-loops and other single-stranded regions of CsrB, CAGGA(U/A/C)G, is a plausible CsrA-binding element. |
| Links | |
| Keywords |
Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Base Sequence; Cloning, Molecular; Escherichia coli; Escherichia coli Proteins; Haemophilus influenzae; Models, Molecular; Molecular Sequence Data; Nucleic Acid Conformation; Pectobacterium carotovorum; RNA/metabolism; RNA, Long Untranslated; RNA, Untranslated; RNA-Binding Proteins/genetics; RNA-Binding Proteins/metabolism; Recombinant Proteins/metabolism; Repetitive Sequences, Nucleic Acid; Repressor Proteins/metabolism; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization |
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