PMID:9197421
| Citation |
Membrillo-Hernandez, J, Cook, GM and Poole, RK (1997) Roles of RpoS (sigmaS), IHF and ppGpp in the expression of the hmp gene encoding the flavohemoglobin (Hmp) of Escherichia coli K-12. Mol. Gen. Genet. 254:599-603 |
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| Abstract |
The Escherichia coli K-12 gene hmp encodes the flavohemoglobin Hmp. A hmp promoter phi(hmp-lacZ)-operon fusion was constructed in the chromosome and its activity measured during the growth cycle. Logarithmically growing cultures had low levels of phi(hmp-lacZ) expression, which increased two-fold at the onset of stationary phase in rich medium. The effect was abolished in a strain carrying a null allele of the gene rpoS encoding the stationary phase-specific sigma subunit of RNA polymerase sigmaS. A himA mutation resulted in a 1.5-fold increase in expression of phi(hmp-lacZ) but did not affect growth phase-dependent regulation. A single transcriptionial start site was found for hmp, located 38 bp upstream of the initiation codon. Putative Fnr boxes at positions -2 to +11 occur in the hmp promoter region. |
| Links | |
| Keywords |
Amino Acid Sequence; Bacterial Proteins/genetics; Bacterial Proteins/physiology; Base Sequence; Carrier Proteins; Cyclic AMP Receptor Protein/physiology; Dihydropteridine Reductase; Escherichia coli/genetics; Escherichia coli/growth & development; Escherichia coli Proteins; Gene Expression Regulation, Bacterial/genetics; Guanosine Tetraphosphate/physiology; Hemeproteins/genetics; Integration Host Factors; Molecular Sequence Data; Mutation; NADH, NADPH Oxidoreductases; Recombinant Fusion Proteins; Sigma Factor/genetics; Sigma Factor/physiology; Transcription, Genetic/genetics |
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