PMID:9167969
| Citation |
Anderson, DG, Churchill, JJ and Kowalczykowski, SC (1997) Chi-activated RecBCD enzyme possesses 5'-->3' nucleolytic activity, but RecBC enzyme does not: evidence suggesting that the alteration induced by Chi is not simply ejection of the RecD subunit. Genes Cells 2:117-28 |
|---|---|
| Abstract |
Homologous recombination in Escherichia coli is initiated by the RecBCD enzyme, and is stimulated by DNA elements known as Chi (chi) sites. The RecBCD enzyme is both a helicase and a nuclease. Recognition of chi causes both attenuation of the 3'-->5' exonuclease activity of the RecBCD enzyme, and activation of an exonuclease activity with 5'-->3' polarity, while leaving the helicase activity unaffected. A variety of evidence suggests that chi-recognition by RecBCD enzyme is accompanied by ejection of the RecD subunit. |
| Links | |
| Keywords |
DNA/metabolism; DNA Helicases/genetics; DNA Helicases/metabolism; DNA, Single-Stranded/metabolism; Endodeoxyribonucleases/genetics; Endodeoxyribonucleases/metabolism; Enzyme Activation; Escherichia coli Proteins; Exodeoxyribonuclease V; Exodeoxyribonucleases/genetics; Exodeoxyribonucleases/metabolism; Regulatory Sequences, Nucleic Acid |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.