PMID:9159874
| Citation |
Ishchenko, AA, Bulychev, NV, Maksakova, GA, Johnson, F and Nevinsky, GA (1997) Recognition and conversion of single- and double-stranded oligonucleotide substrates by 8-oxoguanine-DNA glycosylase from Escherichia coli. Biochemistry Mosc. 62:204-11 |
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| Abstract |
The substrate specificity of 8-oxoguanine-DNA glycosylase was studied. The data showed for the first time that the enzyme can cleave single-stranded deoxyoligonucleotides containing an 8-oxoG link. The values of K(m) and Vmax for a range of single-stranded and double-stranded oligonucleotides (23 bp) containing 8-oxoG at different positions of one chain. These parameters consistently depended on the position of 8-oxoG in single-stranded or double-stranded substrates. A possible mechanism responsible for substrate recognition by 8-oxoguanine-DNA glycosylase is described. |
| Links | |
| Keywords |
Base Sequence; DNA-Formamidopyrimidine Glycosylase; Escherichia coli/genetics; Escherichia coli Proteins; Kinetics; N-Glycosyl Hydrolases/metabolism; Oligodeoxyribonucleotides/chemistry; Substrate Specificity |
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