PMID:9150238
| Citation |
Sanders, OI, Rensing, C, Kuroda, M, Mitra, B and Rosen, BP (1997) Antimonite is accumulated by the glycerol facilitator GlpF in Escherichia coli. J. Bacteriol. 179:3365-7 |
|---|---|
| Abstract |
In a search for genes responsible for the accumulation of antimonite in Escherichia coli, TnphoA was used to create a pool of random insertional mutants, from which one antimonite-resistant mutant was isolated. Sequence analysis showed that the TnphoA insertion was located in the glpF gene, coding for the glycerol facilitator GlpF. The mutant was shown to be defective in polyol transport by GlpF. These results suggest that in solution Sb(III) is recognized as a polyol by the glycerol facilitator. |
| Links | |
| Keywords |
Alkaline Phosphatase/genetics; Antimony/metabolism; Aquaporins; Bacterial Outer Membrane Proteins/genetics; Bacterial Outer Membrane Proteins/metabolism; DNA Transposable Elements; Drug Resistance, Microbial; Escherichia coli/drug effects; Escherichia coli/genetics; Escherichia coli/growth & development; Escherichia coli Proteins; Genes, Bacterial; Glycerol/metabolism; Mutagenesis, Insertional |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.