PMID:9144799
| Citation |
Schmitt, E, Fromant, M, Plateau, P, Mechulam, Y and Blanquet, S (1997) Crystallization and preliminary X-ray analysis of Escherichia coli peptidyl-tRNA hydrolase. Proteins 28:135-6 |
|---|---|
| Abstract |
Peptidyl-tRNA hydrolase from Escherichia coli, a monomer of 21 kDa, was overexpressed from its cloned gene pth and crystallized by using polyethylene glycol as precipitant. The crystals are orthorhombic and have unit cell parameters a = 47.24 A, b = 63.59 A, and c = 62.57 A. They belong to space group P2(1)2(1)2(1) and diffract to better than 1.2 A resolution. The structure is being solved by multiple isomorphous replacement. |
| Links | |
| Keywords |
1-Propanol; Carboxylic Ester Hydrolases/chemistry; Carboxylic Ester Hydrolases/isolation & purification; Cell Line; Crystallization; Crystallography, X-Ray; Electrophoresis, Polyacrylamide Gel; Escherichia coli/chemistry; Escherichia coli/enzymology; Polyethylene Glycols |
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