PMID:9112442

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Citation

Zou, C, Thomas, MS, Keen, J and Glass, RE (1997) A nested set of C-terminal deletions of the alpha subunit of Escherichia coli RNA polymerase define regions concerned with assembly, proteolysis, stabilization and transcriptional activation in vivo. Genes Cells 2:81-94

Abstract

The alpha subunit of eubacterial RNA polymerase comprises an N-terminal assembly domain and a mobile C-terminal domain which provides an activation contact site for class I transcription activators. One particular C-terminal alpha mutant, rpoA341, impairs the response of Escherichia coli RNA polymerase to several activators, including MelR.

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Keywords

Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Binding Sites; DNA-Directed RNA Polymerases/genetics; DNA-Directed RNA Polymerases/immunology; DNA-Directed RNA Polymerases/metabolism; Enzyme Stability; Escherichia coli/enzymology; Genetic Complementation Test; Precipitin Tests; Recombinant Proteins/genetics; Recombinant Proteins/metabolism; Sequence Deletion; Transcriptional Activation

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