PMID:9111035

Citation	Snyders, S, Ramamurthy, V and Oliver, D (1997) Identification of a region of interaction between Escherichia coli SecA and SecY proteins. J. Biol. Chem. 272:11302-6
Abstract	SecA ATPase promotes Escherichia coli protein translocation by its association with the preprotein or preprotein-SecB complex, anionic phospholipids, and the other core component of translocase, integral membrane protein SecYEG. Using ligand affinity blotting we demonstrate a direct interaction of SecA with SecY protein. Proteolysis and gene truncation or fusion studies were used to further define this interaction. Our results demonstrate that the carboxyl-terminal third of SecA protein binds to the amino-terminal 107 amino acid residues of SecY protein. The direct demonstration of these interactions culminate studies that have inferred an interaction between SecA and SecYEG, and they are consistent with studies suggesting that this region of SecA interacts with the inner membrane.
Links	PubMed
Keywords	Adenosine Triphosphatases/genetics; Adenosine Triphosphatases/metabolism; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Bacterial Proteins/secretion; Binding Sites; Biological Transport; Escherichia coli/metabolism; Escherichia coli Proteins; Membrane Transport Proteins; Protein Binding; Protein Precursors/metabolism; Recombinant Fusion Proteins/metabolism; Sequence Deletion
edit table

Significance

You can help EcoliWiki by summarizing why this paper is useful

You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.

Add links to pages that link here (e.g. gene, product, method pages)

See Help:References for how to manage references in EcoliWiki.