PMID:9103983
| Citation |
Henderson, IR, Meehan, M and Owen, P (1997) Antigen 43, a phase-variable bipartite outer membrane protein, determines colony morphology and autoaggregation in Escherichia coli K-12. FEMS Microbiol. Lett. 149:115-20 |
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| Abstract |
The product controlling colony form variation and autoaggregation in Escherichia coli K-12 (the flu gene product) has been identified as the phase-variable, bipartite, outer membrane protein, termed antigen 43 (Ag43). Identification is based: (i) on complete correlation in authentic flu variants between colony morphology/autoaggregation and Ag43 expression as determined by colony and Western immunoblotting and immunofluorescence microscopy; and (ii) on the use of a specific probe to map the gene encoding Ag43 to a position (min 43) on the E. coli chromosome previously established for flu. |
| Links | |
| Keywords |
Amino Acid Sequence; Antibody Specificity; Antigens, Bacterial/genetics; Antigens, Bacterial/immunology; Antigens, Bacterial/isolation & purification; Bacterial Adhesion/physiology; Bacterial Outer Membrane Proteins/genetics; Bacterial Outer Membrane Proteins/immunology; Bacterial Outer Membrane Proteins/isolation & purification; Blotting, Southern; Blotting, Western; DNA Probes; Escherichia coli/chemistry; Escherichia coli/cytology; Escherichia coli/growth & development; Fluorescent Antibody Technique; Gene Expression Regulation, Bacterial/physiology; Immunoblotting; Molecular Sequence Data; Polymerase Chain Reaction |
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