PMID:9079931
| Citation |
Klee, SR, Tzschaschel, BD, Timmis, KN and Guzman, CA (1997) Influence of different rol gene products on the chain length of Shigella dysenteriae type 1 lipopolysaccharide O antigen expressed by Shigella flexneri carrier strains. J. Bacteriol. 179:2421-5 |
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| Abstract |
Introduction of the rol genes of Shigella dysenteriae 1 and Escherichia coli K-12 into Shigella flexneri carrier strains expressing the heterologous S. dysenteriae type 1 lipopolysaccharide resulted in the formation of longer chains of S. dysenteriae 1 O antigen. In bacteria producing both homologous and heterologous O antigen, this resulted in a reduction of the masking of heterologous O antigen by homologous lipopolysaccharide and an increased immune response induced by intraperitoneal immunization of mice by recombinant bacteria. The rol genes of S. dysenteriae 1 and E. coli K-12 were sequenced, and their gene products were compared with the S. flexneri Rol protein. The primary sequence of S. flexneri Rol differs from both E. coli K-12 and S. dysenteriae 1 Rol proteins only at positions 267 and 270, which suggests that this region may be responsible for the difference in biological activities. |
| Links | |
| Keywords |
Amino Acid Sequence; Animals; Bacterial Proteins/genetics; Bacterial Vaccines/genetics; Cloning, Molecular; Escherichia coli Proteins; Genes, Bacterial; Mice; Molecular Sequence Data; O Antigens/chemistry; Sequence Alignment; Sequence Homology, Amino Acid; Shigella dysenteriae/genetics; Shigella dysenteriae/immunology; Shigella flexneri; Vaccines, Synthetic/genetics |
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