PMID:9063459
| Citation |
Tran, QH, Bongaerts, J, Vlad, D and Unden, G (1997) Requirement for the proton-pumping NADH dehydrogenase I of Escherichia coli in respiration of NADH to fumarate and its bioenergetic implications. Eur. J. Biochem. 244:155-60 |
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| Abstract |
In Escherichia coli the expression of the nuo genes encoding the proton pumping NADH dehydrogenase I is stimulated by the presence of fumarate during anaerobic respiration. The regulatory sites required for the induction by fumarate, nitrate and O2 are located at positions around -309, -277, and downstream of -231 bp, respectively, relative to the transcriptional-start site. The fumarate regulator has to be different from the O2 and nitrate regulators ArcA and NarL. For growth by fumarate respiration, the presence of NADH dehydrogenase I was essential, in contrast to aerobic or nitrate respiration which used preferentially NADH dehydrogenase II. The electron transport from NADH to fumarate strongly decreased in a mutant lacking NADH dehydrogenase I. The mutant used acetyl-CoA instead of fumarate to an increased extent as an electron acceptor for NADH, and excreted ethanol. Therefore, NADH dehydrogenase I is essential for NADH-->fumarate respiration, and is able to use menaquinone as an electron acceptor. NADH-->dimethylsulfoxide respiration is also dependent on NADH dehydrogenase I. The consequences for energy conservation by anaerobic respiration with NADH as a donor are discussed. |
| Links | |
| Keywords |
Acetates/metabolism; Anaerobiosis; Dimethyl Sulfoxide/pharmacology; Electron Transport; Electron Transport Complex I; Energy Metabolism/drug effects; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli/growth & development; Escherichia coli/metabolism; Ethanol/metabolism; Fumarates/metabolism; Fumarates/pharmacology; Gene Expression Regulation, Bacterial/drug effects; NAD/metabolism; NADH, NADPH Oxidoreductases/genetics; NADH, NADPH Oxidoreductases/metabolism; Proton Pumps/metabolism |
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