PMID:9048558
| Citation |
Nguyen, LH and Burgess, RR (1997) Comparative analysis of the interactions of Escherichia coli sigma S and sigma 70 RNA polymerase holoenzyme with the stationary-phase-specific bolAp1 promoter. Biochemistry 36:1748-54 |
|---|---|
| Abstract |
We have investigated the interactions of Escherichia coli sigma 70 and sigma S holoenzyme RNA polymerases (E sigma S and E sigma 70) with the stationary-phase-specific bolAp1 promoter by various footprinting methods in vitro. E sigma S and E sigma 70 have been shown to transcribe the bolApl promoter in vitro. We have determined the effects of salt and holoenzyme concentrations on E sigma S and E sigma 70 open complex formation at the bolAp1 promoter in vitro. We have obtained a high-resolution hydroxyl radical (OH.) footprint of E sigma S and E sigma 70 on the bolApl promoter. The OH. footprinting data show remarkable similarities between the footprints of the heparin-resistant transcription complexes of the two holoenzymes which have the same +1 transcription start site. However, there are distinctive differences in the protection patterns in the region between -20 and -10 of the bolAp1 promoter. KMnO4 reactivity assays reveal that, at 37 degrees C, both holoenzymes produced similar but not identical patterns of reactivities. |
| Links |
PubMed Online version:10.1021/bi961175h |
| Keywords |
Base Sequence; DNA Footprinting; DNA-Directed RNA Polymerases/genetics; DNA-Directed RNA Polymerases/metabolism; Deoxyribonuclease I/metabolism; Escherichia coli/enzymology; Escherichia coli/genetics; Genes, Bacterial; Hydroxyl Radical/metabolism; Molecular Sequence Data; Potassium Permanganate/metabolism; Promoter Regions, Genetic; Sigma Factor/genetics; Sigma Factor/metabolism; Transcription, Genetic |
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