PMID:9019401
| Citation |
Stark, H, Orlova, EV, Rinke-Appel, J, Jünke, N, Mueller, F, Rodnina, M, Wintermeyer, W, Brimacombe, R and van Heel, M (1997) Arrangement of tRNAs in pre- and posttranslocational ribosomes revealed by electron cryomicroscopy. Cell 88:19-28 |
|---|---|
| Abstract |
The three-dimensional structure of the translating 70S E. coli ribosome is presented in its two main conformations: the pretranslocational and the posttranslocational states. Using electron cryomicroscopy and angular reconstitution, structures at 20 A resolution were obtained, which, when compared with our earlier reconstruction of "empty" ribosomes, showed densities corresponding to tRNA molecules--at the P and E sites for posttranslocational ribosomes and at the A and P sites for pretranslocational ribosomes. The P-site tRNA lies directly above the bridge connecting the two ribosomal subunits, with the A-site tRNA fitted snugly against it at an angle of approximately 50 degrees, toward the L7/L12 side of the ribosome. The E-site tRNA appears to lie between the side lobe of the 30S subunit and the L1 protuberance. |
| Links | |
| Keywords |
Binding Sites; Escherichia coli; Image Processing, Computer-Assisted/methods; Microscopy, Electron/methods; Models, Molecular; Polyribosomes/ultrastructure; Protein Biosynthesis; RNA, Transfer/ultrastructure; Ribosomes/ultrastructure |
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