PMID:9016619
| Citation |
Nègre, D, Bonod-Bidaud, C, Oudot, C, Prost, JF, Kolb, A, Ishihama, A, Cozzone, AJ and Cortay, JC (1997) DNA flexibility of the UP element is a major determinant for transcriptional activation at the Escherichia coli acetate promoter. Nucleic Acids Res. 25:713-8 |
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| Abstract |
The specific interaction of the upstream element-containing promoter of the Escherichia coli acetate operon with either the RNA polymerase holoenzyme or its alpha subunit has been analyzed by the base removal method. Our results indicate that: (i) direct and specific base contacts can be detected in the acetate promoter-alpha subunit complex; (ii) base elimination in the upstream element of the acetate promoter enhances the binding of RNA polymerase. A similar effect is observed when studying the interactions between RNA polymerase and the rrnB ribosomal operon P1 promoter. |
| Links | |
| Keywords |
Acetates/metabolism; Base Sequence; Binding Sites/genetics; DNA, Bacterial/metabolism; DNA-Directed RNA Polymerases/metabolism; Escherichia coli/genetics; Genes, Bacterial; Molecular Sequence Data; Promoter Regions, Genetic; Transcription, Genetic; Transcriptional Activation; rRNA Operon |
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