PMID:9015366

Citation	Jung, YH and Lee, Y (1997) Escherichia coli rnpB promoter mutants altered in stringent response. Biochem. Biophys. Res. Commun. 230:582-6
Abstract	The promoter of the rnpB gene (encoding the RNA component of Escherichia coli RNase P) shares a consensus discriminator sequence, located between the -10 hexamer sequence and the transcription start site, with other promoters whose activities are repressed upon stringent condition. Under stringent conditions induced by seryl-tRNA starvation the transcription of the rnpB gene was repressed in wild type E. coli but not in a relaxed strain carrying a relA- mutation. Site-directed mutagenesis was carried out to examine sequences of the rnpB promoter necessary for stringent control. The results indicate that the discriminator region is responsible for the transcription repression of the rnpB gene during the stringent response and that both the content and position of GC pairs in the region determine the strength of negative stringent signals.
Links	PubMed Online version:10.1006/bbrc.1996.6005
Keywords	Endoribonucleases/genetics; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli Proteins; Gene Expression Regulation, Bacterial; Gene Expression Regulation, Enzymologic; Genes, Bacterial; Mutagenesis, Site-Directed; Promoter Regions, Genetic; RNA, Catalytic/genetics; Ribonuclease P; Sequence Deletion; Transcription, Genetic
edit table

Significance

You can help EcoliWiki by summarizing why this paper is useful

You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.

Add links to pages that link here (e.g. gene, product, method pages)

See Help:References for how to manage references in EcoliWiki.