PMID:9013822

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Citation

Miura, K, Tomioka, Y, Suzuki, H, Yonezawa, M, Hishinuma, T and Mizugaki, M (1997) Molecular cloning of the nemA gene encoding N-ethylmaleimide reductase from Escherichia coli. Biol. Pharm. Bull. 20:110-2

Abstract

Using the gene mapping membrane technique, we identified a gene (nemA) that encodes N-ethylmaleimide reductase in Escherichia coli. The open reading frame encodes a polypeptide of 365 amino acids with a molecular mass of 39,514 Da. The deduced amino acid sequence showed a high degree of homology (87% identical) with the pentaerythritol tetranitrate reductase of Enterobacter cloacae and the morphinone reductase of Pseudomonas putida (52% identical).

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Keywords

Amino Acid Sequence; Base Sequence; Cloning, Molecular; Escherichia coli/genetics; Ethylmaleimide/metabolism; Genes, Bacterial; Molecular Sequence Data; Oxidoreductases/chemistry; Oxidoreductases/genetics

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