PMID:9006053

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Citation

Haussmann, C, Rohdich, F, Lottspeich, F, Eberhardt, S, Scheuring, J, Mackamul, S and Bacher, A (1997) Dihydroneopterin triphosphate epimerase of Escherichia coli: purification, genetic cloning, and expression. J. Bacteriol. 179:949-51

Abstract

The enzyme catalyzing the epimerization at position 2' of dihydroneopterin triphosphate was purified by a factor of about 10,000 from cell extract of Escherichia coli. The cognate gene was cloned, sequenced, expressed, and mapped to kb 2427 on the E. coli chromosome.

Links

PubMed PMC178780

Keywords

Amino Acid Sequence; Bacterial Proteins/genetics; Base Sequence; Cloning, Molecular; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli Proteins; Genes, Bacterial; Molecular Sequence Data; Neopterin/analogs & derivatives; Pteridines/metabolism; Racemases and Epimerases/genetics; Racemases and Epimerases/isolation & purification; Restriction Mapping; Sequence Analysis, DNA; Sequence Homology, Amino Acid

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