PMID:9006053
Citation |
Haussmann, C, Rohdich, F, Lottspeich, F, Eberhardt, S, Scheuring, J, Mackamul, S and Bacher, A (1997) Dihydroneopterin triphosphate epimerase of Escherichia coli: purification, genetic cloning, and expression. J. Bacteriol. 179:949-51 |
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Abstract |
The enzyme catalyzing the epimerization at position 2' of dihydroneopterin triphosphate was purified by a factor of about 10,000 from cell extract of Escherichia coli. The cognate gene was cloned, sequenced, expressed, and mapped to kb 2427 on the E. coli chromosome. |
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Keywords |
Amino Acid Sequence; Bacterial Proteins/genetics; Base Sequence; Cloning, Molecular; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli Proteins; Genes, Bacterial; Molecular Sequence Data; Neopterin/analogs & derivatives; Pteridines/metabolism; Racemases and Epimerases/genetics; Racemases and Epimerases/isolation & purification; Restriction Mapping; Sequence Analysis, DNA; Sequence Homology, Amino Acid |
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