PMID:9003535

Citation	Babic, I, Andrew, SE and Jirik, FR (1996) MutS interaction with mismatch and alkylated base containing DNA molecules detected by optical biosensor. Mutat. Res. 372:87-96
Abstract	An optical biosensor was used to monitor interactions between the Escherichia coli DNA mismatch repair molecule MutS and various immobilized oligonucleotides. While associating poorly with single-stranded DNA, MutS was capable of rapid association/dissociation from homoduplex DNA. The interaction of MutS with oligonucleotide 30-mers containing single site mismatches demonstrated that during the dissociation phase, MutS binding was greatest to a G-G mismatch, followed by G-T > A-A > C-T, A-C. Binding to A-G, T-T and C-C mispairs was marginally higher than that seen between MutS and homoduplex DNA. The ability of MutS to interact with 30-mers containing alkylated bases was also tested. While binding to O6-methyl-G-C, or to O4-methyl-T-A base pairs was similar to that of homoduplex DNA, strong binding was seen to a O6-methyl-G-T mispair. O4-methyl-T-G, however, was poorly recognized by MutS, with relative binding affinity similar to homoduplex DNA, predicting poor in vivo recognition of O4-methyl-T-G by MutS. Interestingly, MutS demonstrated a relatively high affinity for an 1,N6-etheno-A-T containing homoduplex. Thus, in allowing rapid evaluation of interactions between such molecules, the biosensor will be useful to structure-function analyses.
Links	PubMed Online version:10.1016/S0027-5107(96)00170-4
Keywords	Adenosine Triphosphatases; Alkylation; Bacterial Proteins/chemistry; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Base Composition; Biosensing Techniques; DNA, Single-Stranded/chemistry; DNA, Single-Stranded/metabolism; DNA-Binding Proteins; Deoxyadenine Nucleotides/chemistry; Deoxyadenine Nucleotides/metabolism; Deoxyguanine Nucleotides/chemistry; Deoxyguanine Nucleotides/metabolism; Escherichia coli Proteins; MutS DNA Mismatch-Binding Protein; Nucleic Acid Heteroduplexes/chemistry; Nucleic Acid Heteroduplexes/metabolism; Oligonucleotides/chemistry; Oligonucleotides/genetics; Oligonucleotides/metabolism; Recombinant Proteins/chemistry; Recombinant Proteins/genetics; Recombinant Proteins/metabolism
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