PMID:8973315
| Citation |
Reizer, J, Reizer, A, Merrick, MJ, Plunkett, G 3rd, Rose, DJ and Saier, MH Jr (1996) Novel phosphotransferase-encoding genes revealed by analysis of the Escherichia coli genome: a chimeric gene encoding an Enzyme I homologue that possesses a putative sensory transduction domain. Gene 181:103-8 |
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| Abstract |
Two genes (ptsI and ptsA) that encode homologues of the energy coupling Enzyme I of the phosphoenolpyruvate-dependent sugar-transporting phosphotransferase system (PTS) have previously been identified on the Escherichia coli chromosome. We here report the presence of a third E. coli gene, designated ptsP, that encodes an Enzyme I homologue, here designated Enzyme INtr. Enzyme INtr possesses an N-terminal domain homologous to the N-terminal domains of NifA proteins [(127 amino acids (aa)] joined via two tandem flexible linkers to the C-terminal Enzyme I-like domain (578 aa). Structural features of the putative ptsP operon, including transcriptional regulatory signals, are characterized. We suggest that Enzyme INtr functions in transcriptional regulation of nitrogen-related operons together with previously described PTS proteins encoded within the rpoN operon. It may thereby provide a link between carbon and nitrogen assimilatory pathways. |
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| Keywords |
Animals; Bacterial Proteins/classification; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Binding Sites/genetics; Cricetinae; DNA-Binding Proteins; DNA-Directed RNA Polymerases/genetics; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli Proteins; Genome, Bacterial; Molecular Sequence Data; Multigene Family; Operon; Phosphoenolpyruvate Sugar Phosphotransferase System/chemistry; Phosphoenolpyruvate Sugar Phosphotransferase System/classification; Phosphoenolpyruvate Sugar Phosphotransferase System/genetics; Phosphotransferases (Nitrogenous Group Acceptor)/chemistry; Phosphotransferases (Nitrogenous Group Acceptor)/classification; Phosphotransferases (Nitrogenous Group Acceptor)/genetics; Phylogeny; RNA Polymerase Sigma 54; Recombinant Fusion Proteins/genetics; Regulatory Sequences, Nucleic Acid; Salmonella typhimurium/enzymology; Sequence Homology, Amino Acid; Sigma Factor/genetics; Transcription Factors/metabolism; Transcription, Genetic |
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