PMID:8968081

Citation	Martin, FL, Carmichael, PL, Crofton-Sleigh, C, Venitt, S, Phillips, DH and Grover, PL (1996) Genotoxicity of human mammary lipid. Cancer Res. 56:5342-6
Abstract	We tested the proposition that human mammary lipid contains mutagenic/genotoxic agents that could cause DNA damage in adjacent epithelial cells. Lipid samples from breast tissue surgically removed from 40 women undergoing elective reduction mammoplasty were extracted by a solid-phase procedure. Mutagenicity was observed in Salmonella typhimurium TA98 and TA1538 in 16 of 40 (40%) extracts assayed with rat-liver S9, but not in its absence. No mutagenicity was seen in S. typhimurium TA100 or Escherichia coli WP2uvrA(pKM101). Bacterial mutagenicity correlated with micronucleus-forming activity in a metabolically competent mammalian cell line (MCL-5). This genotoxic activity merits further investigation in relation to the etiology of breast cancer.
Links	PubMed
Keywords	Adipose Tissue/chemistry; Adolescent; Adult; Animals; Biotransformation; Breast/chemistry; Breast Neoplasms/etiology; Carcinogens, Environmental/pharmacokinetics; Carcinogens, Environmental/toxicity; Cell Line; Escherichia coli/drug effects; Escherichia coli/genetics; Female; Humans; Lipid Peroxidation; Lipids/isolation & purification; Lipids/toxicity; Male; Malondialdehyde/analysis; Malondialdehyde/pharmacology; Micronucleus Tests; Microsomes, Liver/metabolism; Middle Aged; Mutagenicity Tests; Oils/pharmacology; Oxidation-Reduction; Rats; Rats, Wistar; Salmonella typhimurium/drug effects; Salmonella typhimurium/genetics; Solubility
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