PMID:8955123
| Citation |
Spahn, CM, Remme, J, Schäfer, MA and Nierhaus, KH (1996) Mutational analysis of two highly conserved UGG sequences of 23 S rRNA from Escherichia coli. J. Biol. Chem. 271:32849-56 |
|---|---|
| Abstract |
The 23 S-type rRNA contains two phylogenetically conserved UGG sequences, which have the potential to bind the universal CCA-3'-ends of tRNAs at the ribosomal peptidyltransferase center by base pairing. The first two positions, UG, of these sequences at the helix-loop 80 (U2249G2250) and helix-loop 90 (Psi2580G2581) and some related nucleotides were tested by site-directed mutagenesis for their involvement in ribosomal function, i.e. peptidyltransferase. The plasmid-derived mutated 23 S rRNA comprised about 50% of the total 23 S rRNA. None of the single mutations caused an assembly defect, and all 50 S subunits carrying an altered 23 S rRNA could freely exchange with the pools of 70S ribosomes and polysomes. The mutations at the helix-loop 80 region hardly affected bacterial growth. However, mutations at the helix 90 caused severe growth effects and severely impaired the in vitro protein synthesis, showing that this 23 S rRNA region is of high importance for ribosomal function. |
| Links | |
| Keywords |
Base Sequence; DNA Mutational Analysis; Escherichia coli/genetics; Molecular Sequence Data; Nucleic Acid Conformation; Polyribosomes/metabolism; Protein Biosynthesis; RNA, Ribosomal, 23S/genetics; RNA, Transfer/metabolism; RNA, Transfer, Phe/metabolism; Ribosomes/metabolism; Ribosomes/ultrastructure; Structure-Activity Relationship |
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