PMID:8931336
| Citation |
McNicholas, PM, Chiang, RC and Gunsalus, RP (1996) The Escherichia coli modE gene: effect of modE mutations on molybdate dependent modA expression. FEMS Microbiol. Lett. 145:117-23 |
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| Abstract |
The Escherichia coli modABCD operon, which encodes a high-affinity molybdate uptake system, is transcriptionally regulated in response to molybdate availability by ModE. Here we describe a highly effective enrichment protocol, applicable to any gene with a repressor role, and establish its application in the isolation of transposon mutations in modE. In addition we show that disruption of the ModE C-terminus abolishes derepression in the absence of molybdate, implying this region of ModE controls the repressor activity. Finally, a mutational analysis of a proposed molybdate binding motif indicates that this motif does not function in regulating the repressor activity of ModE. |
| Links | |
| Keywords |
Bacterial Proteins; DNA Mutational Analysis; Enzyme Activation; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli Proteins; Gene Expression Regulation, Bacterial/drug effects; Gene Expression Regulation, Bacterial/physiology; Genes, Bacterial/physiology; Genes, Regulator/physiology; Malate Dehydrogenase/genetics; Molecular Sequence Data; Molybdenum/metabolism; Molybdenum/pharmacology; Mutation/physiology; Operon/physiology; Plasmids; Transcription Factors/genetics |
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