PMID:8915528
| Citation |
Raynal, LC, Krisch, HM and Carpousis, AJ (1996) Bacterial poly(A) polymerase: an enzyme that modulates RNA stability. Biochimie 78:390-8 |
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| Abstract |
We have constructed a strain that overexpresses E coli poly(A) polymerase (PAP I). The recombinant protein was soluble, and a partially purified extract had high levels of poly(A) polymerising activity. An antiserum raised against the overexpressed PAP I has permitted two types of analysis: the identification of other E coli proteins that may interact with PAP I, and the search for PAP I-like proteins in other bacteria. Immunoprecipitation experiments suggest that PAP I is associated with a 48-kDa protein. This protein remains to be identified. Western blotting using the antiserum against E coli PAP I revealed related proteins in a variety of Gram-negative bacteria and in B subtilis. A comparison of the E coli protein with putative poly(A) polymerases recently identified in H influenza and B subtilis showed highly conserved sequences in the amino terminal and central portions of the proteins that may be important for enzyme activity. |
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| Keywords |
Amino Acid Sequence; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Blotting, Western; Conserved Sequence; Escherichia coli/enzymology; Gene Expression Regulation, Bacterial/genetics; Molecular Sequence Data; Plasmids/genetics; Polynucleotide Adenylyltransferase/metabolism; Precipitin Tests; RNA/metabolism; RNA-Binding Proteins; Recombinant Proteins/genetics; Sequence Homology, Nucleic Acid |
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