PMID:8913496
| Citation |
Akimitsu, N, Mizushima, T, Suzuki, E, Miki, T and Sekimizu, K (1996) Growth phenotypes of Escherichia coli carrying a mutation of acidic phospholipid synthesis. Biol. Pharm. Bull. 19:1275-8 |
|---|---|
| Abstract |
We identified novel phenotypes of a pgsA3 mutant lacking the potential to synthesize phosphatidylglycerophosphate, a precursor of phosphatidylglycerol. The first phenotype is limited cell growth at a high temperature, under the condition of low salt. The phenotype was co-transduced with a phenotype lacking the potential to synthesize phosphatidylglycerol in a P1 transduction experiment, and was restored by transformation with a plasmid containing a wild type pgsA gene. The second phenotype of the pgsA3 mutant was resistant to growth in the presence of a low concentration of kanamycin (4 micrograms/ml). P1 transduction and transformation with the plasmid containing the wild-type pgsA gene revealed that the pgsA3 mutation was also responsible for the second phenotype. |
| Links | |
| Keywords |
Escherichia coli/genetics; Kanamycin/pharmacology; Mutagenesis; Phenotype; Phosphatidylglycerols/biosynthesis; Plasmids/metabolism; Temperature; Transferases (Other Substituted Phosphate Groups)/biosynthesis |
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