PMID:8900067
| Citation |
Lorenz, E and Stauffer, GV (1996) MetR-mediated repression of the glyA gene in Escherichia coli. FEMS Microbiol. Lett. 144:229-33 |
|---|---|
| Abstract |
Inactivation of either of the two MetR binding sites centered at bp -143 and 121 relative to the +1 transcription start site results in reduced glyA-lacZ expression in a wild-type strain below the level seen in a metR mutant. This reduced expression is dependent on the side of the DNA helix MetR binds relative to the RNA polymerase binding site. Thus, a single MetR dimer bound to the DNA may play a physiological role in maintaining appropriate glyA gene expression, functioning as a repressor under low MetR conditions. |
| Links | |
| Keywords |
Bacterial Proteins/genetics; Bacterial Proteins/physiology; Enzyme Induction; Escherichia coli/genetics; Gene Expression Regulation, Bacterial; Glycine Hydroxymethyltransferase/biosynthesis; Glycine Hydroxymethyltransferase/genetics; Methionine/metabolism; Mutagenesis; Promoter Regions, Genetic/genetics; Recombinant Fusion Proteins/metabolism; Repressor Proteins/genetics; Repressor Proteins/physiology; Trans-Activators/genetics; Trans-Activators/physiology |
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