PMID:8895496
| Citation |
Matijasevic, Z, Stering, A, Niu, TQ, Austin-Ritchie, P and Ludlum, DB (1996) Release of sulfur mustard-modified DNA bases by Escherichia coli 3-methyladenine DNA glycosylase II. Carcinogenesis 17:2249-52 |
|---|---|
| Abstract |
The toxic effects of sulfur mustard have been attributed to DNA modification with the formation of 7-hydroxyethylthioethyl guanine, 3-hydroxyethylthioethyl adenine and the cross-link, di-(2-guanin-7-yl-ethyl)sulfide. To investigate the action of bacterial 3-methyladenine DNA glycosylase II (Gly II) on these adducts, calf thymus DNA was modified with [14C]sulfur mustard and used as a substrate for Gly II. Gly II releases both 3-hydroxyethylthioethyl adenine and 7-hydroxyethylthioethyl guanine from this substrate. In comparison with the activity of Gly II towards methylated DNA, 3-hydroxyethylthioethyl adenine is released somewhat more slowly than 3-methyladenine, while 7-hydroxyethylthioethyl guanine is released much more readily than 7-methylguanine. Glycosylase action may play a role in protecting cells from the toxic effects of sulfur mustard. |
| Links | |
| Keywords |
DNA Adducts/metabolism; DNA Glycosylases; DNA Repair; Escherichia coli/enzymology; Mustard Gas; N-Glycosyl Hydrolases/metabolism; Substrate Specificity |
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