PMID:8892832
| Citation |
Harms, N, Ras, J, Reijnders, WN, van Spanning, RJ and Stouthamer, AH (1996) S-formylglutathione hydrolase of Paracoccus denitrificans is homologous to human esterase D: a universal pathway for formaldehyde detoxification? J. Bacteriol. 178:6296-9 |
|---|---|
| Abstract |
Downstream of flhA, the Paracoccus denitrificans gene encoding glutathione-dependent formaldehyde dehydrogenase, an open reading frame was identified and called fghA. The gene product of fghA showed appreciable similarity with human esterase D and with the deduced amino acid sequences of open reading frames found in Escherichia coli, Haemophilus influenzae, and Saccharomyces cerevisiae. Mutating fghA strongly reduced S-formylglutathione hydrolase activity. The mutant was unable to grow on methanol and methylamine, indicating that the enzyme is essential for methylotrophic growth. S-Formylglutathione hydrolase appears to be part of a formaldehyde detoxification pathway that is universal in nature. |
| Links | |
| Keywords |
Adenosine Triphosphatases; Carboxylesterase; Carboxylic Ester Hydrolases/metabolism; Endopeptidase Clp; Formaldehyde/toxicity; Humans; Molecular Sequence Data; Mutation; Paracoccus denitrificans/enzymology; Paracoccus denitrificans/genetics; Sequence Homology, Amino Acid; Serine Endopeptidases/genetics; Thiolester Hydrolases/genetics; Thiolester Hydrolases/metabolism |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.