PMID:8892824
| Citation |
Bogachev, AV, Murtazina, RA and Skulachev, VP (1996) H+/e- stoichiometry for NADH dehydrogenase I and dimethyl sulfoxide reductase in anaerobically grown Escherichia coli cells. J. Bacteriol. 178:6233-7 |
|---|---|
| Abstract |
Anaerobically grown Escherichia coli cells were shown to acidify the reaction medium in response to oxygen or dimethyl sulfoxide (DMSO) pulses, with the H+/e- stoichiometry being close to 2.5 and 1.5, respectively. In the presence of the NADH dehydrogenase I (NDH-I) inhibitor 8-methyl-N-vanillyl-6-nonenamide (capsaicin) or in mutants lacking NDH-I, this ratio decreased to 1 for O2 and to 0 for DMSO. These data suggest that (i) the H+/e- stoichiometry for E. coli NDH-I is at least 1.5 and (ii) the DMSO reductase does not generate a proton motive force. |
| Links | |
| Keywords |
Anaerobiosis; Dimethyl Sulfoxide/pharmacology; Electron Transport Complex I; Electrons; Escherichia coli/drug effects; Escherichia coli/enzymology; Escherichia coli/growth & development; Iron-Sulfur Proteins; NADH, NADPH Oxidoreductases/antagonists & inhibitors; NADH, NADPH Oxidoreductases/metabolism; Oxidoreductases/metabolism; Oxygen; Protons |
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