PMID:8858155

Citation	Malhotra, A, Severinova, E and Darst, SA (1996) Crystal structure of a sigma 70 subunit fragment from E. coli RNA polymerase. Cell 87:127-36
Abstract	The 2.6 A crystal structure of a fragment of the sigma 70 promoter specificity subunit of E. coli RNA polymerase is described. Residues involved in core RNA polymerase binding lie on one face of the structure. On the opposite face, aligned along one helix, are exposed residues that interact with the -10 consensus promoter element (the Pribnow box), including four aromatic residues involved in promoter melting. The structure suggests one way in which DNA interactions may be inhibited in the absence of RNA polymerase and provides a framework for the interpretation of a large number of genetic and biochemical analyses.
Links	PubMed
Keywords	Amino Acid Sequence; Conserved Sequence; Crystallography, X-Ray; DNA, Bacterial/chemistry; DNA, Bacterial/genetics; DNA, Bacterial/metabolism; DNA-Directed RNA Polymerases/chemistry; DNA-Directed RNA Polymerases/genetics; DNA-Directed RNA Polymerases/metabolism; Escherichia coli/enzymology; Models, Molecular; Molecular Sequence Data; Mutation; Nucleic Acid Denaturation; Promoter Regions, Genetic/genetics; Protein Binding; Protein Conformation; Sigma Factor/chemistry; Sigma Factor/genetics; Sigma Factor/metabolism
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