PMID:8841524
| Citation |
Shida, T, Iwasaki, H, Shinagawa, H and Kyogoku, Y' (1995) Structural requirement of four-way junction DNA for binding to and cleavage by RuvC. Nucleic Acids Symp. Ser. ':5-6 |
|---|---|
| Abstract |
Sixteen bimobile four-way junction DNAs were designed to clarify the consensus sequence at the resolution site by the E. coli RuvC protein. The consensus sequence is 5'-T decreases X-3' (X: A, G, C, and T) without exception, but the sequences at the cleaved sites are not necessarily symmetric. The presence of symmetrically located two AT base-pairs at the junction is the minimal requirement for resolution. |
| Links | |
| Keywords |
Bacterial Proteins/metabolism; Base Composition; Base Sequence; Binding Sites; Consensus Sequence; DNA, Bacterial/chemistry; DNA, Bacterial/genetics; DNA, Bacterial/metabolism; Endodeoxyribonucleases/metabolism; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins; Molecular Structure |
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