PMID:8837474

Citation	Saleh, AZ, Yamanaka, K, Niki, H, Ogura, T, Yamazoe, M and Hiraga, S (1996) Carboxyl terminal region of the MukB protein in Escherichia coli is essential for DNA binding activity. FEMS Microbiol. Lett. 143:211-6
Abstract	The purified MukB protein of Escherichia coli has DNA binding activity and nucleotide binding activity. We have isolated a mutation, mukB1013, causing a substitution of valine at position 1379 to leucine. This mutant MukB protein was defective for DNA binding, while the ATP binding activity remained unaffected. A truncated MukB protein that is short of 109 amino acids from the C-terminus failed to bind DNA.
Links	PubMed
Keywords	Adenosine Triphosphate/metabolism; Bacterial Proteins/chemistry; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Base Sequence; Binding Sites/genetics; Chromosomal Proteins, Non-Histone; DNA Primers/genetics; DNA-Binding Proteins/chemistry; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins; Mutagenesis, Site-Directed; Phenotype; Plasmids/genetics; Point Mutation; Sequence Deletion; Temperature
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