PMID:8824224
| Citation |
Johansson, M and von Heijne, G (1996) Membrane topology of Kch, a putative K+ channel from Escherichia coli. J. Biol. Chem. 271:25912-5 |
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| Abstract |
We have mapped the topology of the C-terminal half of the putative potassium channel protein Kch in the inner membrane of Escherichia coli using PhoA fusions. Our results are consistent with the widely assumed six-transmembrane helix model for eukaryotic voltage-gated ion channels and place both ends of the proposed channel-lining P-segment on the periplasmic side of the inner membrane. The rather hydrophobic P-segment is found to translocate only slowly across the inner membrane and seems to be near a threshold for stop-transfer function. |
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| Keywords |
Amino Acid Sequence; Electrophoresis, Polyacrylamide Gel; Endopeptidase K/metabolism; Escherichia coli/chemistry; Escherichia coli Proteins; Kinetics; Models, Chemical; Molecular Sequence Data; Potassium Channels/chemistry; Potassium Channels/metabolism; Protein Conformation; Recombinant Fusion Proteins/metabolism |
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