PMID:8801422
| Citation |
Wissenbach, U, Six, S, Bongaerts, J, Ternes, D, Steinwachs, S and Unden, G (1995) A third periplasmic transport system for L-arginine in Escherichia coli: molecular characterization of the artPIQMJ genes, arginine binding and transport. Mol. Microbiol. 17:675-86 |
|---|---|
| Abstract |
A new binding-protein-dependent transport system of Escherichia coli specific for L-arginine was characterized by genetic and biochemical means. The system is encoded by five adjacent genes, artPIQMJ (art standing for arginine transport), which are organized in two transcriptional units (artPIQM and artJ). The artl and artJ gene products (Artl and ArtJ) are periplasmic binding proteins with sequence similarity to binding proteins for polar (basic) amino acids. The artQ, artM and artP products are similar to the transmembraneous proteins and the ATPase of binding-protein-dependent carriers. The mature Artl and J proteins were localized in the periplasm and lacked signal peptides of 19 amino acid residues. Artl and ArtJ were isolated from overproducing strains. ArtJ specifically binds L-arginine with high affinity and overproduction of ArtJ stimulated L-arginine uptake by the bacteria. The substrate for Artl is not known, and isolated Artl did not bind common amino acids, various basic uncommon amino acids or amines. It is concluded that the artPIQM artJ genes encode a third arginine-uptake system in addition to the known argT hisJQMP system of Salmonella typhimurium and E. coli and the arginine (-ornithine) carrier (aps) of E. coli. |
| Links | |
| Keywords |
Amino Acid Sequence; Amino Acid Transport Systems, Basic; Arginine/metabolism; Bacterial Proteins/genetics; Bacterial Proteins/isolation & purification; Base Sequence; Biological Transport; Carrier Proteins/genetics; Carrier Proteins/isolation & purification; Cell Compartmentation; Cell Fractionation; Cloning, Molecular; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins; Genes, Bacterial; Genomic Library; Membrane Proteins/genetics; Membrane Proteins/isolation & purification; Molecular Sequence Data; Periplasmic Binding Proteins; Promoter Regions, Genetic; Restriction Mapping; Sequence Analysis; Sequence Homology, Amino Acid |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.
